sheep anti cd169 primary antibody  (R&D Systems)

Journal: Physiological Reports

Article Title: Water intake regulates mucosal immunity in rat jejunal villi via IL ‐1β, IL ‐6, and IL ‐10

doi: 10.14814/phy2.70891

Figure Lengend Snippet: (a) Representative immunoreactivities CD68 of a marker of macrophage in the lamina propria of rat jejunal villi without (control) and with the clodronate treatment. (b) Representative immunoreactivities of F4/80, a marker of macrophage in the lamina propria of rat jejunal villi without (control) and with the clodronate treatment. (c) Representative immunoreactivities of CD169, a marker of macrophage in the lamina propria of rat jejunal villi without (control) and with the clodronate treatment. (d–f) Summarized data of density analyses of CD69, F4/80, and CD169, respectively. The error bars represent SDs.

Article Snippet: The fixed samples were sectioned, and the slices were washed three times with PBS and incubated for 2 h at room temperature with primary polyclonal antibodies to FITC‐labeled CD68 (catalog no. bs‐06449R, Bioss, Boston, USA), F4/80 (catalog no. 28463‐1‐AP, Proteintech, Rosemont, USA), or CD169 (catalog no. bs‐10751R, Bioss, Boston, USA), all diluted to 1:5000.

Techniques: Marker, Control

Journal: Biological Research for Nursing

Article Title: Estrogen Stabilizes Interferon-Inducible Genes During Skeletal Muscle Regeneration

doi: 10.1177/10998004251411301

Figure Lengend Snippet: CD169+ macrophages in regenerating areas at 96-h and 192-h postinjury. CD169+ macrophages in the regenerating area of plantarflexor muscle group cross-sections at 96-h (A–D) and 192-h (E–H) postinjury. The regenerating area (black box) in each hematoxylin and eosin (H and E)-stained cross section (A, C, E, G) is matched to an area (black box) in a serial section stained with an anti-CD169 antibody (B, D, F, H) to detect the CD169 protein coded by Siglec1. h, hours postinjury

Article Snippet: The primary antibody was a purified rat anti-mouse CD169 antibody (AbD Serotec-Bio-Rad, 1:20 dilution, Hercules, CA; or Biolegend, 1:50 dilution, San Diego, CA).

Techniques: Staining